Journal: Cell Reports

Article Title: Notch Signaling Mediates Secondary Senescence

doi: 10.1016/j.celrep.2019.03.104

Figure Lengend Snippet:

Article Snippet: IMR90 normal human diploid fibroblasts , ATTC , ATCC Cat# CRL-7931, RRID:CVCL_0347.

Techniques: Plasmid Preparation, Recombinant, cDNA Synthesis, Imaging, Multiplex Assay, Software

Journal: Toxicology and applied pharmacology

Article Title: Role of the PI3K/Akt pathway in cadmium induced malignant transformation of normal prostate epithelial cells.

doi: 10.1016/j.taap.2020.115308

Figure Lengend Snippet: Cell phenotype and tumor formation in nude mice. Parental (RWPE1/PWR1E) and cadmium-exposed (Cd-RWPE1/Cd-PWR1E) cells under the reflected light microscope (A). Sketch showing injection scheme (B). Tumor formation by Cd-RWPE1 cells (C-D). Batch matched parental RWPE1 cells did not result in any tumors (D). Tumor volume over the course of experiment (D). There were four replicates for each cell line. Error bars ±SE.

Article Snippet: Human prostate epithelial cell lines and their culture Human normal prostate epithelial cell lines RWPE1 and PWR1E were purchased from American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Light Microscopy, Injection

Journal: Toxicology and applied pharmacology

Article Title: Role of the PI3K/Akt pathway in cadmium induced malignant transformation of normal prostate epithelial cells.

doi: 10.1016/j.taap.2020.115308

Figure Lengend Snippet: Wound healing assay. Percent artificial wound closure after 24 hours (A; G). Representative pictures of the artificial wound at 0 and 24 hours of cell culture (B; H). Error bar ±SD. Chemotactic cell migration and invasion assay. Average percent migration (C; I) and invasion (E; K) in Cd-exposed (Cd-RWPE1 and Cd-PWR1E) cells compared to their normal parental (RWPE1 and PWR1E) controls. Data are represented as the mean ±SD from at least three replicates. Representative pictures of migration (D; J) and invasion (F; L) in both cell lines.

Article Snippet: Human prostate epithelial cell lines and their culture Human normal prostate epithelial cell lines RWPE1 and PWR1E were purchased from American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Wound Healing Assay, Cell Culture, Migration, Invasion Assay

Journal: Toxicology and applied pharmacology

Article Title: Role of the PI3K/Akt pathway in cadmium induced malignant transformation of normal prostate epithelial cells.

doi: 10.1016/j.taap.2020.115308

Figure Lengend Snippet: Differential expression by RT2 profiler array, real-time PCR and immunoblot analyses of the PI3K/Akt signaling pathway. Scatter plot representing the array results. Upregulated genes are indicated by red points and downregulated genes are in green (A). Heat Map shows the fold changes in expression of 84 PI3K/Akt pathway genes in Cd-RWPE1 vs. RWPE1 cells (B). The complete gene names, location on array and fold changes are indicated in Supplemental Table 1. TaqMan gene expression (C-I) and immunoblot (J) analyses of selected PI3K/Akt pathway genes. Graphical representation of the relative fold upregulation in protein levels based on the average intensity of the target proteins in all replicates and normalized to their endogenous controls (K). Error bars ±SD. Experiments were performed three times and each time there were three replicates in TaqMan assays. For RT2 profiler array RNA from three biological replicates was pooled together for the cDNA synthesis for all four cell lines.

Article Snippet: Human prostate epithelial cell lines and their culture Human normal prostate epithelial cell lines RWPE1 and PWR1E were purchased from American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Quantitative Proteomics, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Gene Expression, cDNA Synthesis

Journal: Toxicology and applied pharmacology

Article Title: Role of the PI3K/Akt pathway in cadmium induced malignant transformation of normal prostate epithelial cells.

doi: 10.1016/j.taap.2020.115308

Figure Lengend Snippet: Significantly up- (> 2 fold) and down-regulated ( <0.5) genes in Cd exposed (Cd-RWPE1; Cd-PWR1E) compared to parental normal (RWPE1; PWR1E) cells.

Article Snippet: Human prostate epithelial cell lines and their culture Human normal prostate epithelial cell lines RWPE1 and PWR1E were purchased from American Type Culture Collection (ATCC) (Manassas, VA).

Techniques:

Journal: Toxicology and applied pharmacology

Article Title: Role of the PI3K/Akt pathway in cadmium induced malignant transformation of normal prostate epithelial cells.

doi: 10.1016/j.taap.2020.115308

Figure Lengend Snippet: Cd-RWPE1 is denoted as “1” and parental normal RWPE1 as “0” in the plots. Five overlapping pathways are enriched in Cd-RWPE1 vs. Parental RWPE1 as control. These pathways are KEGG_Prostate cancer Pathway (A). KEGG_Apoptosis (B). KEGG_MAPK (C). KEGG_Pathways in cancer (D). KEGG_ERBB (E) pathways. Same pathways were enriched in Cd-PWR1E vs. normal parental PWR1E cells (Supplemental Figures 1 and 2).

Article Snippet: Human prostate epithelial cell lines and their culture Human normal prostate epithelial cell lines RWPE1 and PWR1E were purchased from American Type Culture Collection (ATCC) (Manassas, VA).

Techniques: Control